Cayman’s Transglutaminase Activity Assay Kit provides a convenient method of detecting transglutaminase activity in cell lysates and tissue homogenates. In this assay, transglutaminase transfers an amine group from hydroxylamine (amine donor, Transglutaminase Assay Substrate 2) to a glutamine residue of a peptide substrate (amine acceptor, Transglutaminase Assay Substrate 1). The resulting glutamyl-hydroxamate-containing product forms a chromogenic complex with ferric iron (Fe3+), which is readily detectable at 525 nm. With our Transglutaminase Assay Substrate 1, transglutaminases have a reaction rate that is 16-fold faster when compared to the traditional amine acceptor, Z-Gln-Gly, as the substrate. Thus, Cayman’s Transglutaminase Activity Assay kit allows for achieving significantly higher signals in a shorter, 20-minute reaction time. Furthermore, the Transglutaminase Assay Stop Solution is specially formulated to prevent the aggregation of proteins in most samples, allowing users to avoid a centrifugation step that is required in many competitors’ assay kits.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
