Ac-Nle-Pro-Nle-Asp-AMC

Ac-Nle-Pro-Nle-Asp-AMC is a specific substrate for 26S proteasome. Ac-Nle-Pro-Nle-Asp-AMC can be used for the 26S proteasome caspase-like activity analysis[1][2][3].

[1] Dunlop RA, et al. The impact of specific oxidized amino acids on protein turnover in J774 cells. Biochem J. 2008 Feb 15;410(1):131-40. DOI:10.1042/BJ20070161
[2] Kirk-Ballard H, et al. An extract of Artemisia dracunculus L. inhibits ubiquitin-proteasome activity and preserves skeletal muscle mass in a murine model of diabetes. PLoS One. 2013;8(2):e57112. DOI:10.1371/journal.pone.0057112
[3] Pakavathkumar P, et al. Methylene Blue Inhibits Caspases by Oxidation of the Catalytic Cysteine. Sci Rep. 2015 Sep 24;5:13730. DOI:10.1038/srep13730