Synaptosomal-associated protein 25 (SNAP-25) is a member of the SNARE complex, which also includes syntaxin and VAMP, that is responsible for fusing synaptic vesicles with the presynaptic plasma membrane to facilitate neurotransmitter release.1 Two SNAP-25 isoforms, SNAP-25a and SNAP-25b, are generated through alternative splicing, with SNAP-25b expressed only during the postnatal period and as the predominant isoform in the brain.2 SNAP-25 contains two α helices, as well as one large and several smaller intrinsically disordered domains.1 SNAP-25 is located primarily on the intracellular side of the presynaptic plasma membrane in neurons and interacts with a variety of proteins to orchestrate vesicle fusion in a calcium-triggered manner and to mediate spine development.1 It is also found in the pancreas, enteroendocrine cells, and the chromaffin cells of the adrenal medulla where it is involved in hormone secretion.2 SNAP-25 can be phosphorylated at serine 187 (Ser187) in a neuronal activity-dependent manner by PKC, a modification that increases the rate of synaptic vesicle recruitment and is essential for SNAP-25b inhibition of voltage-gated calcium channels (VGCCs) and incorporation of NMDA receptors into the postsynaptic membrane.3,4,5 Phosphorylation at Ser187 also facilitates neurotransmitter release and inhibits presynaptic short-term plasticity via regulation of synaptic vesicle dynamics.6 A point mutation at Ser187 (S187A) induces anxiety-like behavior in mice homozygous for the mutation and induces working memory deficits and an immature phenotype in hippocampal dental granule cells of adult mice heterozygous for the mutation.7,8 Cayman’s SNAP-25 (Phospho-Ser187) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes SNAP-25 (phospho-Ser187) at approximately 25 kDa from mouse and rat samples.WARNING This product is not for human or veterinary use.
