D-Alanine Sofosbuviris a useful reagent for synthesizingβ-D-2''-Deoxy-2''-α-fluoro-2''-β-C-methyluridine nucleotide that can selectively inhibit Hepatitis C virus (HCV) NS5B polymerase, which is a potential target for developing therapies for HCV.D-Alanine Sofosbuviris also an impurity of Sofosbuvir (P839640).