D-Alanine Sofosbuvir?is a useful reagent for synthesizing?β-D-2''-Deoxy-2''-α-fluoro-2''-β-C-methyluridine nucleotide that can selectively inhibit Hepatitis C virus (HCV) NS5B polymerase, which is a potential target for developing therapies for HCV.?D-Alanine Sofosbuvir?is also an impurity of Sofosbuvir (P839640).