Prostaglandin F Synthase (human recombinant)

Human prostaglandin F synthase (PGFS) is a ~37 kDa monomeric protein with high sequence similarity to aldo-keto reductase family members. It exhibits two related primary activities, catalyzing the isomerization of PGH₂ to PGF_2α (PGH₂ 9,11-endoperoxide reductase activity), as well as the formation of 11β-PGF_2α from PGD₂ (PGD₂ 11-ketoreductase activity) in the presence of NADPH. The enzyme also exhibits reductase activities toward carbonyl compounds such at nitroacetophenone, nitrobenzaldehyde, and 9,10-phenanthrene quinine (PQ). Cayman’s human recombinant PGFS has been expressed and purified from an E.coli overexpression system. Enzyme activity was determined by monitoring the change in absorbance at 340 nm using PGD₂ as the substrate and NADPH as the cofactor (PGD₂ 11-ketoreductase activity).

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