Prostaglandin E Synthase (cytosolic) Polyclonal Antibody

Prostaglandin E synthase (PGES) catalyzes the isomerization of PGH₂ to PGE₂ . Cytosolic and microsomal PGES enzymes have been cloned and characterized. Cytosolic PGES (cPGES) is a glutathione-dependent enzyme with a predicted size of 18.6 kDa (23 kDa on SDS-PAGE). The enzyme is expressed in a wide variety of tissues and cells, the levels of which are unaffected by treatment with IL-1β and TNF-α. However, enzyme expression increases approximately 3-fold in rat brain following LPS treatment. Microsomal PGES (mPGES) is a 16 kDa protein expressed in a variety of tissues including prostate, testes, and small intestine, as well as in A549 and HeLa cells. In contrast to cPGES, mPGES protein expression is increased in A549 cells following treatment with IL-1β. The two enzymes show <10% homology at the amino acid level.

[1] T TANIOKA. Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis.[J]. The Journal of Biological Chemistry, 2000, 275 42: 32775-32782. DOI: 10.1074/jbc.m003504200
[2] P J JAKOBSSON. Identification of human prostaglandin E synthase: a microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target.[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96 13: 7220-7225. DOI: 10.1073/pnas.96.13.7220
[3] YING SUN. PPARγ Agonist Rosiglitazone Suppresses Renal mPGES-1/PGE2 Pathway in db/db Mice.[J]. PPAR Research, 2013, 2013: 612971. DOI: 10.1155/2013/612971