Anti-RACK1 (N-terminal) antibody is suitable for use in indirect immunofluorescence (1:100-1:200 using paraformaldehyde-fixed HeLa cells), western blot (1:500-1:1,000 using HEK-293T cell lysates) and immunoprecipitation (5-10 μL using HEK-293T cell lysates).
Receptors for activated C-kinase (RACKs) are scaffold proteins for protein kinase C (PKC). It is composed of seven WD repeats, thus resembling the structure of the β subunit of G proteins. It is not a substrate of PKC, but in its presence, the activity of PKC is increased by several fold.
RACK1 is a receptor for activated C kinases that functions downstream of the growth factor receptor signaling pathway. RACK1 also modulates several functions such as cell adhesion, chemotaxis and protrusion. Increased RACK1 expression has been associated with cell migration and metastasis in breast carcinoma. Thus, RACK1 can be used as a prognostic indicator for breast cancer