The purified IgG3 (heavy chain specific) antibody may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. IgG3 (heavy chain specific) antibody was used as secondary antibody in ELISA.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
IgG3 (heavy chain specific) antibody specifically recognizes heavy chain of mouse IgG3. Specificity of the mouse IgG3 is determined by immunodiffusion and IEP techniques. The purified antibody preparation is non-reactive with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM, myeloma proteins.
