Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.1 MBP contains 19 arginine residues, including the arginine residue at position 130 (R130) in human MBP, which corresponds to R128 in the mouse protein, that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.2,1,3 The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.1,4 The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.1 citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.1,4 Cayman's Citrullinated Myelin Basic Protein (R130) Monoclonal Antibody (Clone 3C6) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
