Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 IgG1 production is driven by Th2-stimulated immune responses. IgG1 neutralizes toxins and activates the inhibitory FcγRIIB receptor but does not trigger FcγR-mediated effector functions. Formulations containing humanized, chimeric, or murine IgG1 monoclonal antibodies have been used in the treatment of inflammatory diseases, such as ulcerative colitis, multiple sclerosis, and asthma, as well as cancer.7 Cayman's IgG1 (mouse) Rabbit Monoclonal Antibody (Clone RM106) can be used for ELISA and Western blot (WB) applications. The antibody recognizes the Fc region of IgG1 and detects non-reduced and reduced IgG1 from mouse samples at approximately 150 and 50 kDa, respectively.WARNING This product is not for human or veterinary use.
