Used in cell transport studies, and inhibits deoxyribonuclease I
Actin from bovine muscle has been used as an internal standard and positive control during western blotting.
Actin from rabbit muscle has been used:
- to study its protective role on rabbit muscle lactate dehydrogenase from ascorbate inhibition
- as standard in SDS gel?and immunoblotting
- in the protein pin array assays
Actin is the abundant protein found in many cells which accounts for 10% or more of total protein. Most of the eukaryotic cells shows the presence of actin protein. Outer and inner domains are the two important domains of actin.
Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.