N-[4-[3-[2-Amino-4-(formylamino)-1,6-dihydro-6-oxo-5-pyrimidinyl]-3-oxopropyl]benzoyl]-L-glutamic Acid is an impurity of Pemetrexed (P219550, Disodium Heptahydrate), a chemotherapy drug for the treatment of pleural mesothelioma and non-small cell lung cancer.