sodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate

Product Namesodium 8-amino-5-chloro-1-oxo-7-phenyl-2H-pyrido[3,4-d]pyridazin-4-olate
CAS143556-24-5
MFC13H9ClN4O2.Na
MW311.68
EINECS
MOL File143556-24-5.mol

Chemical Properties

storage temp.	-20°C
solubility	Soluble to 20 mM in water and to 100 mM in DMSO
form	Solid
color	White to yellow
Water Solubility	H2O: 2mg/mL, clear
InChI	InChI=1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
InChIKey	IGEUYSJHQQCEFP-UHFFFAOYSA-M
SMILES	[Na+].C1([O-])=NNC(=O)C2=C(N)C(C3=CC=CC=C3)=NC(Cl)=C12

Safety Information

WGK Germany	WGK 3
Storage Class	11 - Combustible Solids

Usage And Synthesis

Description

L 012 sodium salt is a luminal-based chemiluminescent dye and probe. It is used to detect superoxide in biological systems, specifically in whole inflammatory cells, vascular tissue and blood. It could detect NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS). It is active in vitro and in vivo.

Uses

L 012 Sodium Salt is a chemiluminescent dye used to detect superoxide in biological systems, specifically in whole blood, inflammatory cells, and vascular tissue.

Biological Activity

L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminol, lucigenin and MCLA. Instead of reacting with superoxide anion (O2-) directly, L-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical form, which then reacts with oxygen (O2) to yield O2- and L-012 quinone (q-Lum). The O2- in turn reacts with the LumH· radical, leading eventually to an endoperoxide th at decomposes to emit luminescence. To a less extend, H2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONS, L-012 is also commonly used for screening NADPH oxidase inhibitors.

in vivo

L-012 sodium salt is well distributed in the mouse body and mediates a strong ROS/RNS-dependent luminescent signal in vivo and is useful for monitoring the development and regulation of inflammation in living organisms^[2].

storage

Store at -20°C

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