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NucPE1 features two major visible region absorptions (λ _abs =468 nm, ε=27,300 M ^-1 cm ^-1 ; λ _abs =490 nm, ε=26,000 M ^-1 cm ^-1 ) and a weak emission (λ _em =530 nm, Φ=0.117). Reaction of NucPE1 with H ₂ O ₂ triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 M ^-1 cm ^-1 ) with enhanced emission (λ _em =530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans , where it can respond to subcellular changes in H ₂ O ₂ fluxes.

NucPE1 maintains the ability to selectively target nuclei in vivo . NucPE1 imaging reveals a reduction in nuclear H ₂ O ₂ levels in worms overexpressing sir-2.1 compared to wildtype congeners, supporting a link between this longevity-promoting sirtuin protein and enhanced regulation of nuclear ROS pools.