Serum IgGs against HHV-6 or HHV-7 were detected by Elisa using alkaline phosphatase conjugated goat anti-human IgG F′ab specific at a concentration of 1:1000 diluted in PBS/0.05% skim milk. The antibody was incubated on plates for 2 hours at 37 degrees.
Goat anti-human IgG (gamma-chain specific)-alkaline phosphatase antibody can be used for ELISA applications using serum samples from allergic and borderline hypertensive patients.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific)-Alkaline Phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, and Bence Jones κ, and λ myeloma proteins.
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region aids in the development of therapeutic antibody.
