E. coli?CA has been used for western blotting. It has also been used for lipid monolayer assay.
Cardiolipin is the most important phospholipid of mitochondria. E.coli membrane consists 5-10% of cardiolipin. Cardiolipin is a non-bilayer anionic phospholipid, which has four acyl chains and small headgroup.
Cardiolipin plays a major role in the dynamic organization of membranes and in cell division. It is implicated in proton uptake pathway.