N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-N-(2-(4-(((benzhydryloxy)carbonyl)amino)-2-oxopyrimidin-1(2H)-yl)acetyl)glycine is a peptide nucleic acid (PNA) and can be useful in the detection of circulating microRNA biomarkers from body fluids with application to prostate cancer diagnosis. It can be useful in the study of efficacy of peptide nucleic acid and selected conjugates against specific cellular pathologies of amyotrophic lateral sclerosis. It can also be useful in the study of SNP discrimination by tolane-modified peptide nucleic acids and application for detection of drug resistance in pathogens.