β-Gal-NONOate

β-Gal-NONOate is a nitric oxide (NO) donor which releases NO following activation by β-galactosidase.¹ β-Gal-NONOate is stable in aqueous solution at neutral and acidic pH for several hours, exhibits good water solubility, and is able to cross the cell membrane. Following enzymatic hydrolysis, β-Gal-NONOate decomposes with a half-life of six minutes at pH 5.6.¹ β-Gal-NONOate is cytotoxic to a number of cancer cell-lines and exhibits high bactericidal activity against E. coli transformed with the β-galactosidase gene.^1,2

1. Wu, X., Tang, X., Xian, M., et al. Glycosylated diazeniumdiolates: A novel class of enzyme-activated nitric oxide donors Tetrahedron Lett. 42(23),3779-3782(2001).
2. Chen, C., Shi, Y.Q., Song, J., et al. Delivery of nitric oxide released from β-Gal-NONOate activation by β-galactosidase and its activity against Escherichia coli Biol. Pharm. Bull. 29(6),1239-1241(2006).