Prostaglandin D synthase (PGDS) is a glutathione-dependent enzyme and member of the sigma class of glutathione-S-transferases (GSTs) that catalyzes the conversion of PGH2 (Item No. 17020) to PGD2 (Item No. 12010), an eicosanoid that has numerous biological functions, including vasorelaxation, recruitment of inflammatory cells, and inhibition of platelet aggregation.1,2,3 There are two types of PGDS: lipocalin PGDS (L-PGDS; Item No. 10006788) and hematopoietic PGDS (H-PGDS; Item No. 10006593).3 H-PGDS is found in peripheral tissues and immune cells, including Th2 cells, antigen-presenting cells, mast cells, megakaryocytes, and eosinophils, where it is localized to the cytosol.2 H-PGDS activity is increased by a variety of stimuli, including LPS, anti-IgE antibodies, phorbol 12-myristate 13-acetate (TPA; Item No. 10008014), ionomycin (Item No. 10004974), and inflammatory cytokines such as IL-13, IL-3, or IL-4.3 siRNA silencing of Hpgds decreases LPS-induced production of PGD2 in mouse bone marrow-derived macrophages (BMDMs).4 Transgenic overexpression of HPGDS in mice increases croton oil-induced ear swelling and PGD2 production, and genome-wide deletion of Hpgds exacerbates hypotension and vascular permeability in a mouse model of anaphylaxis.5,6 H-PGDS protein levels are increased in the nasal mucosa of patients with allergic rhinitis, and HPGDS SNPs have been found in individuals with asthma.1,3 Cayman’s Prostaglandin D Synthase (hematopoietic-type; mouse) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes H-PGDS at 23 kDa from human, baboon, mouse, and rat samples.WARNING This product is not for human or veterinary use.
