DIG Easy Hyb can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. DIG Easy Hyb is ready-made, and can be used without any additions. It is specifically designed for use with DIG kits.
DIG Easy Hyb is a nontoxic, ready-to-use hybridization buffer. DIG Easy Hyb does not contain formamide, but facilitates lowering of hybridization temperature comparable to buffers that contain 50% formamide.