RNA Molecular Weight Marker I, DIG-labeled, is used as a size standard in northern blot analysis when the DIG System for Nucleic Acid Labeling and Detection is used.
The digoxigenin-labeled RNA standard can be detected simultaneously with hybridized digoxigenin-labeled probes during the immunological digoxigenin detection reaction, allowing a length determination of the nonradioactively detected RNA on the blot. Either chemiluminescent (e.g., DIG Luminescent Detection Kit) or colorimetric (e.g., DIG Nucleic Acid Detection Kit) detection may be used.
This product is designed as a safer chemical.? The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
