“AQuora® Fluorescein-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein NHS Ester is a xanthene-based dye with photophysical properties comparable to FITC, carboxyfluorescein, and Alexa Fluor® 488 (absorption maximum = 495 nm, emission maximum = 524 nm).This AQuora® Fluorescein NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
