2',3'-Dideoxythymidine-5'-O-(1-thiotriphosphate) (sodium salt)

2',3'-Dideoxythymidine-5'-O-(1-thiotriphosphate) (ddTTP-α-S) is a sulfur-containing nucleoside triphosphate derivative. Thiol-containing dideoxynucleoside triphosphates (α-S-ddNTPs) incorporated into allele-specific primers prevent complete digestion by 5’-phosphodiesterase, which allows for alkylation and identification of SNP-containing alleles.1 ddTTP-α-S has been used for chain extension in PCR assays.2 ddTTP-α-S is a mixture of Rp and Sp isomers.WARNING This product is not for human or veterinary use.

[1] S SAUER. A novel procedure for efficient genotyping of single nucleotide polymorphisms.[J]. Nucleic Acids Research, 2000, 28 5: E13. DOI: 10.1093/nar/28.5.e13
[2] SASCHA SAUER  Richard R  Hans Lehrach. MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging.[J]. Nucleic Acids Research, 2003, 31 11: e63. DOI: 10.1093/nar/gng062