mTORC1 is a protein complex composed of the serine/threonine kinase mammalian target of rapamycin (mTOR), TOR complex subunit LST8 (mLST8), regulatory-associated protein of mTOR (Raptor), proline-rich Akt substrate 40 kDa (PRAS40), and DEP domain-containing mTOR-interacting protein (Deptor) that functions as a nutrient sensor and regulates cell growth and metabolism.1,2,3 PRAS40 and Deptor act as negative regulators of mTORC1 activity, and mLST8 and Raptor as scaffold proteins for substrates such as 4E-BP1 and p70 ribosomal S6 kinase 1 (p70S6K1). mTORC1 localizes to the plasma membrane, lysosome, and nucleus depending on its upstream activation signal, which include AMPK, PI3K, and Akt.4,1 Activation of mTORC1 is influenced by growth factors, energy status, oxygen levels, and amino acid availability and is involved in a wide variety of processes, including aging, autophagy, stem cell and immune function, cellular senescence, and protein translation.1,5,6,3 Dysregulation of the mTOR signaling pathway is associated with various pathologies, including cancer, rheumatoid arthritis, epilepsy, neurodegenerative diseases, and diabetes.1,7,3 Cayman’s mTOR/mLST8/Raptor Complex (human, recombinant) protein can be used for enzyme activity assays.WARNING This product is not for human or veterinary use.
