Cayman’s Caspase-4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human caspase-4, a major upstream effector of non-canonical inflammasome activation. The assay uses a caspase-4-specific fluorogenic substrate, Ac-LEVD-AFC. Caspase-4 cleaves this substrate generating free AFC, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 400 and 505 nm, respectively. The potent and reversible caspase-4 inhibitor AC-LEVD-CHO is included as a positive control.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
