(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮
(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮
英文名称:RA-9
CAS号:919091-63-7
分子式:C19H15N3O5
分子量:365.34
EINECS号:
Mol文件:919091-63-7.mol
(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮 性质
| 熔点 | 230-233 °C(Solv: ethyl ether (60-29-7); acetonitrile (75-05-8)) |
|---|---|
| 沸点 | 588.5±50.0 °C(Predicted) |
| 密度 | 1.414±0.06 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | DMSO：4.17 mg/mL（11.41 mM；超声加热并加热至 80°C） |
| 酸度系数(pKa) | 5.13±0.20(Predicted) |
| 形态 | 固体 |
| 颜色 | 浅黄至黄色 |
(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮 用途与合成方法
RA-9 是一种具有细胞渗透性的蛋白酶体相关的 deubiquitinating enzymes (DUBs) 的强效选择性抑制剂，具有良好的毒性和抗癌活性。RA-9 可在卵巢癌细胞系中选择性地诱导凋亡。
| Target | Value |
|
DUBs
() |
RA-9 (10-30 μM; 48 hours) inhibits growth of ovarian cancer cell lines and primary cultures.
RA-9 (1.25-5 μM; 18 hours) causes cell cycle arrest and caspase-mediated apoptosis in ovarian cancer cells.
RA-9 (5 μM; 0-24 hours) induces ER-stress responses in ovarian cancer cells.
RA-9 (5 μM; over 24 hours) treatment results with time-dependent accumulation of the cleaved formed of PARP noticeable as early as 8 hours.
Cell Viability Assay
| Cell Line: | Cisplatin-sensitive ovarian cancer cell lines TOV-21G and ES-2, Cisplatin-resistant ovarian cancer cell lines HEY and OVCAR-3, primary ovarian cancer cells |
| Concentration: | 10, 20, 30 μM |
| Incubation Time: | 48 hours |
| Result: | Compromised the viability of ovarian cancer cells in a dose-dependent fashion. |
Cell Cycle Analysis
| Cell Line: | ES-2 cells |
| Concentration: | 1.25, 5 μM |
| Incubation Time: | 18 hours |
| Result: | Resulted in a dose-dependent increase in the fraction of ES-2 cells in the G2-M cell cycle phase. |
Western Blot Analysis
| Cell Line: | ES-2, SKOV-3 and TOV-21G ovarian cancer cells |
| Concentration: | 5 μM |
| Incubation Time: | 0-24 h |
| Result: | Caused a time-dependent increase in the steady levels of the early ER-stress marker GRP-78, as well as the late ER-stress markers IRE1-α and Ero1L-α. |
RA-9 (5 mg/kg; i.p; one-day on, two-days off) inhibits human ovarian cancer cell growth in vivo and prolongs survival in a mouse model for ovarian cancer.
| Animal Model: | Six-week-old female immunodeficient (NCr nu/nu) mice |
| Dosage: | 5 mg/kg |
| Administration: | I.p; one-day on, two-days off |
| Result: | Significant reduction in tumor burden at day 12. |
(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-11-08 | S0884 | (3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮 | 919091-63-7 | 5mg | 794.58 |
| 2024-11-08 | S0884 | (3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮 | 919091-63-7 | 25mg | 2432.03 |
(3E,5E)-3,5-双(4-硝基亚苄基)哌啶-4-酮供应商 更多
天津凯利奇生物科技有限公司
联系电话： 15076683720
产品介绍：
英文名称：RA-9
CAS：919091-63-7
纯度：大于98%
包装信息：1g,5g,10g,25g根据客户需要分装
备注：Not For Human Use, Lab Use Only.
上海瀚香生物科技有限公司
联系电话：177-54423994 17754423994
产品介绍：
英文名称：RA-9
CAS：919091-63-7
纯度：98% HPLC LCMS
包装信息：100mg;500mg;1g
备注：现货，仅供科研用途
上海超岚化工科技中心
联系电话：021-QQ：65489617 15618227136
产品介绍：
英文名称：RA-9
CAS：919091-63-7
纯度：98%
包装信息：5MG;10MG;50MG;100MG,1G,5G,100G
上海源溪生物科技有限公司
联系电话：021-58447131 13564518121
产品介绍：
英文名称：RA-9
CAS：919091-63-7
纯度：98% HPLC
包装信息：100mg，250mg，1g
最新发布供应信息
化合物 RA-9|T8464|TargetMol
TargetMol中国（陶术生物）
2024-12-12
aladdin 阿拉丁 R412607 RA-9 919091-63-7 98%
上海阿拉丁生化科技股份有限公司
2024-11-29
