Dihydrofolate reductase is purified by (NH4)2SO4 precipitation, then fractionation on a Sephadex G-75 column, applied to a Blue Sepharose column and eluted with 1mM dihydrofolate. [Al Rubeai & Dole Biochem J 235 301 1986.]
DIHYDROFOLATE REDUCTASE F. BOVINE LIVER, ~8 U/MG, 500 UL Supplier
