Oxysterols are produced through enzymatic or non-enzymatic oxidation of cholesterol.1 They function as metabolic intermediates or bioactive lipids and are involved in the regulation of cholesterol and lipid metabolism and have roles in cell signaling and innate and adaptive immunity, among others. For example, 24(S)-hydroxy cholesterol is important for sterol homeostasis in the CNS, several oxysterols act as liver X receptor (LXR) ligands, and 25-hydroxy cholesterol regulates cholesterol biosynthesis. 25-hydroxy Cholesterol also inhibits replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in vitro and increased rapidly prior to death in the serum of a patient with COVID-19, the primarily respiratory virus caused by SARS-CoV-2.2 Cayman's Oxysterol Derivatization MaxSpec® Kit is designed for the sensitive detection and relative quantitation of oxysterols in biological samples. This is achieved through enzyme-assisted derivatization utilizing d0/d5 labeled Girard's P (GP) reagent to give GP hydrazones. The instrumental response of the derivatized oxysterols is significantly improved due to the presence of a positively charged quaternary nitrogen group in the labeled and unlabeled GP hydrazones. Included in this kit are the essential reagents to derivatize oxysterols in mixtures for sensitive and comparative LC-MS analysis.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
