1,2-Dioleoyl-3(S)-trimethylammoniumpropane (chloride)

1,2-Dioleoyl-3(S)-trimethylammoniumpropane (S-DOTAP) is a cationic lipid and the less immunologically active isomer of DOTAP .^1,2 Lipoplexes containing S-DOTAP and encapsulating siRNA targeting the gene encoding aromatase, CYP19A1, decrease aromatase activity in MCF-7 breast cancer cells.¹ Vaccination with a peptide epitope from human papillomavirus (HPV) 16 protein E7 in S-DOTAP-containing liposomes decrease tumor size but do not induce IFN-γ production by CD8⁺ T cells and tumor-infiltrating lymphocytes (TILs) in a murine cervical cancer model.²

1,2-Dioleoyl-3(S)-trimethylammoniumpropane chloride is a biochemical reagent.

1. Terp, M.C., Bauer, F., Sugimoto, Y., et al. Differential efficacy of DOTAP enantiomers for siRNA delivery in vitro Int. J. Pharm. 430(1-2),328-334(2012).
2. Vasievich, E.A., Chen, W., and Huang, L. Enantiospecific adjuvant activity of cationic lipid DOTAP in cancer vaccine Cancer Immunol. Immunother. 60(5),629-638(2011).