ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been used to coat tissue culture plates for different glioma subtypes and C2C12 muscle cells. It has also been used as a scaffold to increase myotube attachment, maturation and survival.
ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.
The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.
