Ribocil is a synthetic mimic of riboflavin 5’-monophosphate (FMN; ) that competes with the natural ligand to inhibit FMN riboswitch-mediated expression of ribB, a gene responsible for the synthesis and transport of riboflavin. Ribocil depletes cellular levels of riboflavin with an IC50 value of 0.3 μM and inhibits E. coli growth with an MIC value of 2 μg/ml.
