Cy5.5-SE

Product NameCy5.5-SE
CAS442912-55-2
MFC45H47N3O16S4
MW1014.12
EINECS
MOL File442912-55-2.mol

Chemical Properties

solubility	Easily soluble in organic solvents such as DMSO and DMF, very soluble in water
form	Solid
color	Light blue to blue
Appearance	Dark blue solid
ex/em	678/706 nm (PBS buffer)
ε(extinction coefficient)	211000 L⋅mol−1⋅cm−1
Φ(quantum yield)	0.21
InChIKey	DOMDXTIMIZCSNC-UHFFFAOYSA-N
SMILES	CC1(C(N(CCCCCC(=O)ON2C(CCC2=O)=O)C2C=CC3=C(C=C(S(O)(=O)=O)C=C3C1=2)S(O)(=O)=O)=CC=CC=CC1C(C2C3=CC(S(O)(=O)=O)=CC(S([O-])(=O)=O)=C3C=CC=2[N+]=1CC)(C)C)C

Usage And Synthesis

Chemical Properties

Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 Lmol1cm1
Quantum yield (Φ): 0.21

Uses

Cy5.5-SE (Cyanine5.5 NHS ester) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance[2]. Storage: protect from light.

in vivo

Cy5.5-labeled factor VIIa is developed for imagining cancer. Cy5.5 labeled with these targeting proteins specifically localize to the tumor xenografts for at least 14 days but unconjugated Cy5.5 does not localize to any xenografts or organs. This method of imaging anti-tissue factor in the tumor VECs may be useful in detecting primary tumors and metastases as well as monitoring in vivo therapeutic responses^[1]. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin (Cy5.5-Lf-MPNA nanogels) are developed as a promising contrast agent for preoperative MRI and intraoperative fluorescence imaging of glioma^[2].

References

[1] Zhu S, et al. Visualizing cancer and response to therapy in vivo using Cy5.5-labeled factor VIIa and anti-tissue factor antibody. J Drug Target. 2015 Apr;23(3):257-65. DOI:10.3109/1061186X.2014.988217
[2] Ptaszek M. Rational design of fluorophores for in vivo applications. Prog Mol Biol Transl Sci. 2013;113:59-108. DOI:10.1016/B978-0-12-386932-6.00003-X
[3] Shindy, H. A. (2017). Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029
[4] Jiang L, et al. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin for MR and fluorescence imaging of glioma in rats. Biomaterials. 2013 Oct;34(30):7418-28. DOI:10.1016/j.biomaterials.2013.05.078
[5] Lim B, et al. A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology. Theranostics. 2017 Feb 26;7(5):1047-1061. DOI:10.7150/thno.18099

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