MES Hydrate has been used:
- To suspend cells before autophagic induction studies
- In the preparation of Murashige and Skoog growth medium for the growth of Arabidopsis thaliana seedlings
- In the conjugation of hybridization probes to beads before PCR amplification
- To treat fibroblast-derived matrix before conjugation with heparin for use as a vascular endothelial growth factor delivery platform
MES hydrate has been used in the MS (Murashige and Skoog) media for growth of Arabidopsis seedlings. It has been added in the cytoskeleton buffer during immunofluorescence study.
MES hydrate is a zwitterionic N-substituted aminosulfonic acid, known as Good?s buffer, is utilized for many biological applications. It is a useful resolving agent for very small proteins on bis-tris gels. It is mainly used as a biological buffer in plant cell cultures. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.
MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.
