5-Nitro BAPTA is a calcium chelator, combinded with 2-Me-substituted TM ( as a fluorescent moiety), can be used to form a red fluorescent probe (CaTM-2 AM), for imaging of cytoplasmic Ca2+ in cultured living cells. 5-Nitro BAPTA is a building block used in the synthesis of Ca2+ specific chelators, Ca2+ buffers, and fluorescent Ca2+ indicators[1][2].
[1] Takahiro Egawa, et al. Red Fluorescent Probe for Monitoring the Dynamics of Cytoplasmic Calcium Ions. Angew Chem Int Ed. 2013, 52(14):3874-3877.
[2] Jones, et al. Purification and labeling of extracellular vesicles using a mixed mode resin composition: World Intellectual Property Organization, WO2019133842[P]. 2019-07-04.
