NBT/BCIP STOCK SOLUTION

• The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
• Northern blot
• Western blot
• Colony and plaque lifts
• In situ hybridization
• and for applications in immunohistochemistry and immunocytochemistry.

Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C₄₀H₃₀Cl₂N₁₀O₆, BCIP: C₈H₆NO₄BrClP x C₇H₉N

Evaluation: visually