Technical grade IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. Goat IgG was used as negative control antibody in immunohistochemical staining of cecal tissue of pigs at a dilution of 1:56000. Goat IgG was used in immunohistochemistry of rat brain sections at a concentration of 2 μg/ml. The antibody was also used in chromatin immunoprecipitation and as control in infection inhibition assay.
Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents. Goat IgG was used as negative control in immunohistochemical analysis of human heart tissue samples and in preparation of egg-white avidin samples for atomic force microscopy. Goat IgG was used as isotype control antibody in immunohistochemical analysis of tissue sections of human tonsils, colon, small intestine, ileus and spleen.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Goat IgG is purified from normal goat serum by fractionation and ion-exchange chromatography.
