A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2.
Cell permeable: no', 'Primary Target
Nitric oxide (NO) fluorescent indicator', 'Product does not compete with ATP.', 'Reversible: no