The neuronal-specific potassium chloride cotransporter 2 (KCC2) is a member of the SLC12 family of transporters and is encoded by SLC12A5 in humans.1 KCC2 is expressed in mature neurons throughout the CNS and localizes to the soma and dendrite plasma membrane where it mediates chloride ion efflux, maintaining the transmembrane chloride potential.2 It consists of 12 transmembrane helices, which contain an extracellular loop subject to N-glycosylation, as well as intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of KCC2 at threonine 1007 (Thr1007) is mediated by lysine-deficient protein kinase 1 (WNK1) and its effectors, STE20/SPS1-related proline-alanine-rich protein kinase (SPAK) and oxidative stress-responsive kinase 1 (OSR1), resulting in reduced KCC2 activity. Phospho-Thr1007 levels are decreased by the KCC2 activator N-ethylmaleimide (NEM) in HEK293 cells.3 Point mutation of Thr1007 (T1007E) in KCC2 mimics its phosphorylation and abolishes NEM-induced chloride ion efflux in HEK293 cells. Cayman's KCC2 (Phospho-Thr1007) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes KCC2 (phospho-Thr1007) at approximately 135 kDa from mouse and rat samples.WARNING This product is not for human or veterinary use.
