Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 IgG3 production is driven by bacterial- or viral-associated antigens, including HIV-1 and Staphylococcus antigens, and occurs early in the immune response following IgM class-switching.2,7 IgG3 binds to and neutralizes pathogens, as well as activates complement and opsonizes bacteria, leading to complement-dependent cytotoxicity (CDC) and antibody-dependent cell cytotoxicity (ADCC), respectively. Serum IgG3 levels are increased in patients with primary biliary cirrhosis, Sjögren's syndrome, systemic sclerosis, or systemic lupus erythematosus (SLE).8 Cayman's IgG3 (human) Rabbit Monoclonal Antibody - Biotinylated (Clone RM119) can be used for ELISA, immunocytochemistry (ICC), and immunohistochemistry (IHC) applications. The antibody recognizes the heavy chain of IgG3 from human samples.WARNING This product is not for human or veterinary use.
