LNCAP cell lysates were analyzed by western blot using HRP conjugated rabbit anti-goat IgG as the secondary at a 1:7500 dilution in TBSt for 1 hour at room temperature.
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit is suitable for use in immunoblot and immunohistochemistry. The product can also be used for direct ELISA (1:10,000).
IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)–peroxidase antibody can be used as a secondary antibody in ovarian tissue microarray. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with all goat IgG but shows no reactivity with human serum proteins.
Immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reaction.