diSulfo-Cy5 alkyne

Product NamediSulfo-Cy5 alkyne
CAS1617497-19-4
MFC35H42N3NaO7S2
MW703.84
EINECS
MOL File1617497-19-4.mol

Chemical Properties

solubility	Soluble in Water, DMSO, DMF, DCM
Appearance	Dark blue solid
ex/em	649/672 nm (PBS buffer)
ε(extinction coefficient)	271000 L⋅mol−1⋅cm−1
Φ(quantum yield)	0.28
InChIKey	XKVHPAQUKWEXLO-UHFFFAOYSA-N
SMILES	C(N1C(C(C)(C)C2=CC(S(O)(=O)=O)=CC=C12)=CC=CC=CC1=[N+](C2=CC=C(S([O-])(=O)=O)C=C2C1(C)C)C)CCCCC(=O)NCC#C.[NaH]

Usage And Synthesis

Description

Sulfo-Cy5 alkyne is a water soluble cyanine linker containing an alkyne group, which enables Click Chemistry to attach the deeply colored and photostable Cyanine5 fluorophore to various azide-bearing molecules. Sulfo-Cyanine5 is a sulfonated dye with high hydrophilicity and aqueous solubility, high extinction coefficient, good quantum yield, and compatibility with many instruments. This reagent is recommended for conjugation with proteins, nanoparticles, and other applications where hydrophilicity is important.

Chemical Properties

Appearance: Dark blue solid
ex/em: 649/672 nm (PBS buffer)
Extinction coefficient (ε): 271000 Lmol1cm1
Quantum yield (Φ): 0.28

Uses

DiSulfo-Cy5 alkyne is a fluorescent dye can be used as a click chemistry reagent[1].

References

[1] Majouga AG, Dontsova OA, et al. Mixed valence copper(I,II) binuclear complexes with unexpected structure: synthesis, biological properties and anticancer activity. J Med Chem. 2014 Jul 24;57(14):6252-8. DOI:10.1021/jm500154f