Monoclonal Anti-Talin may be used for the localization of talin using various immunochemical assays such as immunoblot, immunocytochemistry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and for microinjection and transfection studies. The antibody recognizes native talin and can be used for immunoprecipitation.
Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the 8d4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Talin is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin.
Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques.
