MEK1, active, GST tagged human has been used to induce kinase reactions in in vitro mitogen activated protein kinase/ERK kinase (MEK)1 kinase assay. It has also been used to activate extracellular-signal-regulated kinase 2 (ERK2) by phosphorylation.
Mitogen activated protein kinase/ERK kinase (MEK)1/2 catalyzes the phosphorylation of tyrosine and then threonine in extracellular-signal-regulated kinase1/2. As an essential component of RAS?RAF?MEK?ERK signaling pathway (MAP kinase signaling cascade), it is involved in the regulation of apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative diseases such as cancer.
