(-)-4,6'-Dimethoxy-6-methyl-3,4'-dioxospiro[benzofuran-2(3H),1'-[2,5]cyclohexadiene]-2'-carboxylic acid methyl ester

Trypacidin is a fungal metabolite originally isolated from A. fumigatus.1 It is active against B. subtilis and M. bovis (MICs = 12.5 and 1.25 μg/ml, respectively), as well as T. cruzi and T. gondii (MICs = 5-10 and 10-20 μg/ml, respectively).1,2 It reduces viability and induces lysis of A549 human lung cancer cells (IC50s = 7.4 μM for both).3 Trypacidin increases survival in a mouse model of T. gondii infection when administered in six doses of 12.5 mg/kg each.1

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1.Balan, J., Ebringer, L., Nemec, P., et al.Antiprotozoal antibiotics. II. Isolation and characterization of trypacidin, a new antibiotic, active against Trypanosoma cruzi and Toxoplasma gondiiJ. Antibiot. (Tokyo)16157-160(1963) 2.Song, Z., Liu, Y., Gao, J., et al.Antitubercular metabolites from the marine-derived fungus strain Aspergillus fumigatus MF029Nat. Prod. Res.1-8(2019) 3.Gauthier, T., Wang, X., Dos Santos, J.S., et al.Trypacidin, a spore-borne toxin from Aspergillus fumigatus, is cytotoxic to lung cellsPLoS One7(2)e29906(2012)