7-Methylguanosine-d3

7-Methylguanosine-d3 is intended for use as an internal standard for the quantification of 7-methylguanosine (Item No. 15988) by GC- or LC-MS. 7-Methylguanosine is a methylated form of the purine nucleoside guanosine (Item No. 27702). It is an RNA modification that is present in the 5’-terminal cap of mRNA, where it promotes translation, as well as in tRNA, where it stabilizes the tRNA structure.1,2 7-Methylguanosine has been used as a substrate to measure the activity of purine nucleoside phosphorylase (PNP).3 Urinary 7-methylguanosine levels are decreased in patients with prostate cancer.1WARNING This product is not for human or veterinary use.

[1] M. A. FERNÁNDEZ-PERALBO. Prostate Cancer Patients–Negative Biopsy Controls Discrimination by Untargeted Metabolomics Analysis of Urine by LC-QTOF: Upstream Information on Other Omics[J]. Scientific Reports, 2016, 6 1. DOI: 10.1038/srep38243
[2] TOMIKAWA C. 7-Methylguanosine Modifications in Transfer RNA (tRNA).[J]. International Journal of Molecular Sciences, 2018, 19 12. DOI: 10.3390/ijms19124080
[3] A. STACHELSKA-WIERZCHOWSKA  J. W. Non-typical nucleoside analogs as fluorescent and fluorogenic indicators of purine-nucleoside phosphorylase activity in biological samples[J]. Analytica Chimica Acta, 2020, 1139: Pages 119-128. DOI: 10.1016/j.aca.2020.09.018