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CLOSTRIPAIN

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Clostripain is isolated from Clostridium histolyticum callogenase by extraction in pH 6.7 buffer, followed by hydroxylapatite chromatography with a 0.1-0.2 M phosphate gradient, then Sephadex G-75 gel filtration with 0.05M phosphate pH 6.7, dialysis and a second hydroxylapatite chromatography (gradient elution with 0.1M 0.3M phosphate, pH 6.7). It has proteinase and esterase activity and is assayed by hydrolysing N-benzoyl-L-arginine methyl ester. [Mitchell & Harrington J Biol Chem 243 4683 1968, Methods Enzymol 19 635 1970.]

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  • Company Name:Shanghai Baoman Biotechnology Co., Ltd.
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  • Products Intro:Product Name:Clostripain
    CAS:9028-00-6
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    Purity:Bio-Ultra Grade+From Clostridium histolyticum, 50 U/mg Package:1mg;5mg Remarks:4054235
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  • Company Name:Shanghai Aladdin Bio-Chem Technology Co.,LTD
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  • Products Intro:Product Name:Clostripain from Clostridium histolyticum
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  • Products Intro:Product Name:Clostripain from Clostridium histolyticum
    CAS:9028-00-6
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