EthidiuM-d5 BroMide
Basic informationMore
- Product Name:EthidiuM-d5 BroMide
- Synonyms: EthidiuM-d5 BroMide 2,7-DiaMino-10- (ethyl-d5)-9-phenylphenanthridiniuM BroMide 2,7-DiaMino-9-phenyl-10-(ethyl-d5) phenanthridiniuM BroMide 2,7-DiaMino-9-phenylphenanthridine-d5 EthobroMide 3,8-DiaMino-5-(ethyl-d5)-6-phenylphenanthridiniuM BroMide DroMilac-d5 HoMidiuM-d5 BroMide
- CAS:
- MF:C21H20BrN3
- MW:394.3076
- EINECS:
- Mol File:Mol File
USE
<p>EthidiuM-d5 BroMide is the labelled ethidium bromide (EtBr). It is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). EthidiuM-d5 Bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.</p>
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