Fluorinert® FC-40 is a colorless, thermally stable, fully- fluorinated liquid. The compound has been used as a high density chase solution in density gradient studies. Fluorinert® FC-40 has been used to assist in the visualization of chromosome segregation of dividing sea urchin embryos. In the direct membrane potential measurement of rat alveolar macrophages the cells were centrifuged into Fluorinert® FC-40.