Cayman’s Nitrotyrosine IP Kit allows for the capture and pulldown of nitrated proteins from cell lysates using a sorbent coupled with a nitrotyrosine monoclonal antibody. The nitrated proteins can be eluted from the resin, loaded to an SDS-PAGE gel, and probed using the included Nitrotyrosine Polyclonal Antibody or a user supplied target specific antibody. A positive control sample assures that the assay is working.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.