Hedgehog acyltransferase-like (HHATL) is the mammalian homolog of yeast Gup1 that is encoded by the HHATL gene in humans. HHATL is a membrane-bound O-acyltransferase (MBOAT) superfamily member and contains multiple transmembrane domains common to this family but lacks acyltransferase function due to a single amino acid substitution of histidine for leucine at position 447. It is localized to the endoplasmic reticulum (ER) and is expressed in the heart, skeletal muscle, and brain. HHATL colocalizes with sonic hedgehog (Shh) and is a negative regulator of Shh N-terminal palmitoylation, a post-translational modification that is critical for Shh signaling in neural development and embryogenesis. Hhatl-/- neonatal mice fail to develop proper suckling ability, leading to malnutrition and death by postnatal day 14. HHATL expression is decreased in six nasopharyngeal carcinoma cell lines, as well as in tissue isolated from patients with nasopharyngeal carcinoma or skin squamous cell carcinoma. Cayman''s HHATL Polyclonal Antibody can be used for flow cytometry (FC), immunofluorescence (IF), and Western blot applications. The antibody recognizes HHATL at ~60 kDa from human samples.
